Simultaneous determination and pharmacokinetic study of P-hydroxybenzaldehyde, 2,3,5,4′-tetrahydroxystilbene-2-O-β-glucoside, emodin-8-O-β-d -glucopyranoside, and emodin in rat plasma by liquid chromatography tandem mass spectrometry after oral administration of Polygonum multiflorum
| Autor: | Yang Zhao, Dan Xue, Hongmei Lin, Longfei Lin, Sali Cao, Boran Ni, Chunjing Yang, Jian Ni |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Polygonum
Chromatography biology General Chemical Engineering Electrospray ionization Extraction (chemistry) Selected reaction monitoring General Engineering biology.organism_classification Analytical Chemistry chemistry.chemical_compound chemistry Pharmacokinetics Oral administration Liquid chromatography–mass spectrometry Emodin |
| Zdroj: | Analytical Methods. 7:244-252 |
| ISSN: | 1759-9679 1759-9660 |
| DOI: | 10.1039/c4ay02197e |
| Popis: | A rapid and specific liquid chromatography tandem mass spectrometry (LC-MS/MS) method was developed for the simultaneous analysis of P-hydroxybenzaldehyde, 2,3,5,4′-tetrahydroxystilbene-2-O-β-glucoside, emodin-8-O-β-D-glucopyranoside, and emodin in rat plasma, and it was applied to the pharmacokinetics (PK) studies of the four compounds from the herb Polygonum multiflorum. The analysis was carried out on a Phenomenex Hydro-RP C18 (150 × 2.0 mm, 4 μm) reversed-phase column by gradient elution with a flow rate of 0.4 mL min−1. All of the analytes including internal standards (I.S.) were monitored by selected reaction monitoring with an electrospray ionization source. Linear responses were obtained for P-hydroxybenzaldehyde, 2,3,5,4′-tetrahydroxystilbene-2-O-β-glucoside, emodin-8-O-β-D-glucopyranoside, and emodin ranging from 1 to 1000 ng mL−1. The intra-day and inter-day precisions (RSD) were less than 8.61% and 8.75%, respectively. The extraction recovery ranged from 77.00 ± 5.54 to 91.30 ± 2.96, and the I.S. was 85.82 ± 3.58%. Stability studies showed that the accuracies of P-hydroxybenzaldehyde, 2,3,5,4′-tetrahydroxystilbene-2-O-β-glucoside, emodin-8-O-β-D-glucopyranoside, and emodin ranged from 94.66 ± 3.54 to 106.84 ± 6.91; the matrix effects ranged from 92.14 ± 3.63 to 103.98 ± 8.71. The validated method was successfully used to determine the concentration–time profiles of P-hydroxybenzaldehyde, 2,3,5,4′-tetrahydroxystilbene-2-O-β-glucoside, emodin-8-O-β-D-glucopyranoside, and emodin. |
| Databáze: | OpenAIRE |
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