The Effect of 30-minute Ischemia on the Levels of IL6, TNFα, NO, Glutathione and Lactic Acid in the Hepatic Tissue of Rats with Hepatic Steatosis

Autor: A. Papalois, Maria Terzopoulou, Argyro Zacharioudaki, Konstantinos Alexiou, Theodore Karatzas, Pagona Kamparoudi, Nikolaos Sikalias, Lamprini Mountzalia
Rok vydání: 2019
Předmět:
Zdroj: Hellenic Journal of Surgery. 91:65-74
ISSN: 1868-8845
0018-0092
DOI: 10.1007/s13126-019-0508-0
Popis: Fatty liver disease (FLD) is becoming increasingly common. Ischemia-reperfusion injury (IRI) is the main pathogenic factor determining morbidity and mortality after major hepatic surgery. This experimental study investigated changes in the levels of interleukin 6 (IL6), tumor necrosis factor α (TNFα), nitric oxide (NO), glutathione (GSH) and lactic acid (LA) in hepatic tissue, in normal rats and rats with severe hepatic steatosis, after 30-minute hepatic ischemia and reperfusion. The aim was to evaluate the ability of the severely steatotic liver to sustain ischemia. The study was conducted on 96 male Wistar rats aged 12–14 weeks, in 48 of which severe hepatic steatosis was induced by administration of a choline-free diet for 12–14 weeks. The other 48 rats received standard laboratory diet for the same period. The effect of 30-minute hepatic ischemia and reperfusion was examined in 24 steatotic and 24 lean livers, which were subjected to hepatic ischemia-reperfusion injury. After 6 and 24 hours of reperfusion, the levels of IL6, TNFα, NO, LA and GSH in the liver tissue of all the rats were measured. The serum levels of aspartate transaminase (AST) and alanine transaminase (ALT) were measured to determine the severity of hepatic injury, and the survival of the rats after surgery was assessed. Rats with steatotic livers subjected to 30-minute hepatic ischemia and reperfusion showed significantly higher liver values of TNFα and LA and significantly lower liver values of IL6, GSH and NO than lean rats. The serum levels of AST and ALT were higher in rats with severely steatotic livers than in those with lean livers, after 30-minute hepatic ischemia and reperfusion, and no rat with a steatotic liver survived surgery. Rats with steatotic livers undergoing 30-minute ischemia and reperfusion show significant elevation of TNFα and LA and reduction in IL6, GSH and NO in liver tissue, with poor survival after surgery, compared to non-steatotic rats. These findings suggest that steatotic livers are more susceptible to ischemia-reperfusion injury than lean livers.
Databáze: OpenAIRE
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