The biosynthesis of13C labeled starch granules as a source of uniformly labeled13C D-glucose

Autor: R. T. Eakin, J. R. Buchholz, C. W. Christenson, E.B. Fowler
Rok vydání: 1973
Předmět:
Zdroj: Journal of Labelled Compounds. 9:443-456
ISSN: 0022-2135
DOI: 10.1002/jlcr.2590090308
Popis: A potato plant has been grown in a closed chamber with CO2 enriched to a level of 31 at. % in 13C as the sole source of carbon. In 80 days the plant produced 1,684 g of tubers labeled with 29 at. % 13C. of the carbon fixed by the potato plant appromixately 70% was found in the tubers while 30% was found in the tops and roots. Recovered starch granules constituted 10% of the fresh weight of these tubers. A 16% yield of glucose was obtained, based on all of the carbon dioxide used. Distribution of the 13C label among the six species of carbon atoms in strach appears to be nearly uniform. The plant and tubers were indistinguishable from those grown on normal isotopic CO2 indicating no gross isotope effect at this level of 13C enrichement.
Databáze: OpenAIRE