The Ocular Protective Effects of Nano/Submicron Particles Prepared from Lycium barbarum Fruits Against Oxidative Stress in an Animal Model
| Autor: | An-I Yeh, Yun-Ju Chen, Nien-Chen Lin, Chang-Hao Yang, Po-Ting Yeh |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
genetic structures Oxidative phosphorylation medicine.disease_cause Andrology Superoxide dismutase 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Paraquat medicine Pharmacology (medical) Pharmacology chemistry.chemical_classification Reactive oxygen species TUNEL assay biology food and beverages eye diseases Ophthalmology 030104 developmental biology Terminal deoxynucleotidyl transferase chemistry Apoptosis 030221 ophthalmology & optometry biology.protein sense organs Oxidative stress |
| Zdroj: | Journal of Ocular Pharmacology and Therapeutics. 36:179-189 |
| ISSN: | 1557-7732 1080-7683 |
| DOI: | 10.1089/jop.2019.0048 |
| Popis: | Purpose: To investigate the antioxidative properties of Lycium barbarum (LB) fruits in the eyes and to study whether LB fruits prepared with new nanotechnology have stronger antioxidative effects. Methods: Fourteen days post-supplementation with milled or blended LB fruits, intravitreal paraquat (PQ) was injected into Wistar rats to create oxidative stress. After an additional 14-day supplementation with LB fruits, the rats were sacrificed. An electroretinogram (ERG) was performed to evaluate retinal function before and after the PQ injection. Expression levels of antioxidative responders' mRNA in retina were detected by reverse transcription-polymerase chain reaction. Superoxide dismutase (SOD) and glutathione reductase activity in the aqueous humor (AqH) were analyzed by ELISA. Immunohistochemistry was conducted to evaluate the morphological changes of retina and the levels of oxidative biomarkers. The levels of cell apoptosis were assessed by the terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay. The reactive oxygen species (ROS) levels in AqH were measured by chemiluminescence methods. Results: The murine eyes supplemented with LB fruits exhibited several changes compared with the control group. The ERGs revealed significant improvement in retinal function. The mRNA expression levels of oxidative responders were downregulated in the retinas. The ROS was significantly reduced in the retinas, but the SOD meaningfully increased in the AqH. Immunohistochemistry staining and TUNEL assays showed decreased incidences of oxidative biomarkers and apoptosis in the retinas. Milled LB fruits exhibited better antioxidative effects than blended fruits. Conclusions: Milled LB fruits demonstrated superior protection against oxidative threats than blended fruits. Thus, these fruits could be an inexpensive supplement for many oxidative stress-related ocular diseases. |
| Databáze: | OpenAIRE |
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