A novel HLA-B*40 allele and novel exon 1 sequences of two B*40 alleles identified in potential marrow donors

Autor:	I.S. Gourley, J. Yee, B.B. Griffith, Malek Kamoun, G.D. Montoya, M. McKeen, Thomas M. Williams, Jane Kearns, Jin Wu, S. Birkos, S. Bassinger
Rok vydání:	2000
Předmět:	Genetics Immunology Locus (genetics) General Medicine Human leukocyte antigen Biology Biochemistry Molecular biology HLA-B law.invention Exon law Genetic variation Immunology and Allergy Allele Oligomer restriction Polymerase chain reaction
Zdroj:	Tissue Antigens. 55:374-377
ISSN:	0001-2815
DOI:	10.1034/j.1399-0039.2000.550413.x
Popis:	Sequence-specific oligonucleotide probe hybridization and sequence-specific primer polymerase chain reaction (PCR) typing suggested the presence of variants of HLA-B*40 in three individuals. Two were part of 3,500 potential marrow donors being screened for the National Marrow Donor Program, while the third was a clinical specimen. PCR products encompassing HLA-B locus exons 1 through 3 were prepared and subcloned. In one individual, a native of the Pacific Islands, sequencing revealed a novel HLA-B*40 allele (B*4023). In two other individuals, a previously unknown exon 1 sequence was determined for HLA-B*4016 (ethnicity unknown) and B*4020 (Hispanic). These findings further illustrate the substantial genetic variation present at the HLA-B locus within human populations ( Note).
Databáze:	OpenAIRE
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