An Improved Radioimmunoassay for α-Melanocyte-Stimulating Hormone (α-MSH) in the Rat: Serum and Pituitary α-MSH Levels after Drugs Which Modify Catecholaminergic Neurotransmission
| Autor: | A.J. Thody, R.J. Penny |
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| Rok vydání: | 1978 |
| Předmět: |
Drug
Catecholaminergic endocrine system medicine.medical_specialty Melanocyte-stimulating hormone integumentary system Endocrine and Autonomic Systems Endocrinology Diabetes and Metabolism media_common.quotation_subject Radioimmunoassay Pharmacology Biology Neurotransmission Cellular and Molecular Neuroscience Endocrinology Hypothalamus Internal medicine medicine Haloperidol hormones hormone substitutes and hormone antagonists Hormone media_common medicine.drug |
| Zdroj: | Neuroendocrinology. 25:193-203 |
| ISSN: | 1423-0194 0028-3835 |
| DOI: | 10.1159/000122741 |
| Popis: | A modification of our previous radioimmunoassay (RIA) for α-melanocyte-stimulating hormone (α-MSH) is described that permits the measurement of circulating levels in the rat without the need for an extraction procedure. Using this method, serum and neurointermediate lobe () immunoreactive α-MSH levels were measured in rats after administration of haloperidol, 2-bromo α-ergocryptine (CB 154), and α-methyl-p-tyrosine (α-MPT). Haloperidol caused a rapid increase, α-MPT a slow increase, and CB 154 a rapid decrease in serum α-MSH. At the time intervals studied none of the drugs had any significant effect on α-MSH content. It was concluded from the results of the above drug treatments that modulation of dopaminergic neurotransmission both pre- and postsynaptically produces changes in serum immunoreactive α-MSH levels. This supports the suggestion that circulating α-MSH in the rat is under an inhibitory control by a catecholaminergic system. |
| Databáze: | OpenAIRE |
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