Development of a nanoparticle microfluidic colour device for point-of-care diagnostics
| Autor: | Peter Pointl, Saied Assadollahi, Thomas Schalkhammer, Roland Palkovits |
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| Rok vydání: | 2011 |
| Předmět: | |
| Zdroj: | International Journal of Design Engineering. 4:159 |
| ISSN: | 1751-5882 1751-5874 |
| Popis: | Through a novel immunoassay nanoparticles (NPs) are captured via immune-reactive proteins and positioned as a sub-monolayer just a few nanometres above a light reflective surface. Here the particles and the mirror form a nanometric interference system with a tuneable colour throughout the full spectrum of light. This concept, which was inspired by nature (‘wings of a butterfly’), creates an intensive colour clearly visible to the eye. By adaptation of this technique point-of-care (POC) tests become more sensitive, specific and time efficient. The novel format employs a combination of microfluidics and ‘resonance-enhanced absorption’ (REA) of gold and palladium nanoparticles quantifying interleukin-6 as an important parameter of acute sepsis. The colour intensity of the rapid test is boosted by using a subsequent silver enhancement. This performed in situ in just two to three minutes and a sensitivity of < 500 pg/ml was obtained. |
| Databáze: | OpenAIRE |
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