An ELISA for recombinant Lepidoglyphus destructor , Lep d 2, and the monitoring of exposure to dust mite allergens in farming households
| Autor: | Eva Johansson, L. H. M. Elfman, M. van Hage-Hamsten, S. Parvaneh |
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| Rok vydání: | 2002 |
| Předmět: |
Allergy
biology business.industry Immunology Aeroallergen medicine.disease_cause biology.organism_classification medicine.disease respiratory tract diseases law.invention Allergen medicine.anatomical_structure immune system diseases law medicine Recombinant DNA biology.protein Mite Immunology and Allergy Antibody business Sensitization Asthma |
| Zdroj: | Clinical & Experimental Allergy. 32:80-86 |
| ISSN: | 0954-7894 |
| Popis: | BACKGROUND Exposure to indoor allergens, such as dust mites, has been recognized as a risk factor for sensitization and symptoms. OBJECTIVE To develop a two-site ELISA for the determination of Lep d 2 in the reservoir, to measure dust mite allergen exposure (Lep d 2, Der p 1, Der f 1 and Der 2) in farm households, and to investigate whether exposure to these allergens is associated with sensitization, asthma and rhinoconjunctivitis. METHODS Monoclonal antibodies to recombinant (r)Lep d 2 were produced with standard hybridoma technique. Dust samples from 393 households were analysed for allergen content by two-site ELISA methods. RESULTS A two-site Lep d 2 ELISA was developed with a detection limit of 0.09 microg/g. The assay was highly reproducible and levels of Lep d 2 showed a strong correlation with the number of Lepidoglyphus mites (r(s): 0.7; P = 0.0002). Lep d 2 was detected in 20% of the homes; levels ranged from 0.09 to 1.7 microg/g of dust. Der p 1 was recorded in 59% of the samples, ranging from 0.055 to 139 microg/g, and Der f 1 and Der 2 in 40% and 50% of the samples, ranging from 0.055 to 24.5 microg/g and 24.3 microg/g, respectively. Dermatophagoides allergens were significantly higher in mattresses than in carpets (P < 0.0001), but this difference was not observed with Lep d 2. A strong relationship between immunoglobulin (Ig)E to rLep d 2 and asthma (OR = 10.4) and rhinoconjunctivitis (OR = 7.5) was seen. Furthermore, sensitization to D. pteronyssinus was significantly associated with asthma (OR: 13.7) and rhinoconjunctivitis (OR: 5.7). CONCLUSION When assessing mite allergen exposure in rural homes, not only the Der p 1, Der f 1 and Der 2 allergens, but also the Lep d 2 allergen should be taken into consideration. |
| Databáze: | OpenAIRE |
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