Genealogical Concordance between Mitochondrial and Nuclear DNAs Supports Species Recognition of the Panamint Rattlesnake (Crotalus mitchellii stephensi)

Autor: Louis W. Porras, Michael E. Douglas, Marlis R. Douglas, Gordon W. Schuett, Blake L. Thomason
Rok vydání: 2007
Předmět:
Zdroj: Copeia. 2007:920-932
ISSN: 1938-5110
0045-8511
Popis: The Speckled Rattlesnake (Crotalus mitchellii) is a polytypic taxon presently composed of five subspecies that range across southwestern North America, including the Baja Peninsula and islands in the Pacific Ocean and Sea of Cortes. The principles of genealogical concordance were employed to test the taxonomic status of three of the five subspecies (C. m. mitchellii, C. m. pyrrhus, and C. m. stephensi). We used two molecular marker systems: mitochondrial (mt) DNA ATPase 8 and 6 genes (675 base pairs, bp), and introns 5 and 6 of the nuclear (n) DNA ribosomal protein (RP) gene (449 bp). These markers were evaluated across 104 individuals of C. mitchellii: C. m. mitchellii (n = 3), C. m. pyrrhus (n = 83), C. m. stephensi (n = 18), with Sistrurus c. catenatus as the distant outgroup. Deep phylogenetic splits were detected in the subspecies of C. mitchellii, with 5.0–6.4% mtDNA sequence divergence (SD) separating C. m. mitchellii and C. m. pyrrhus, while C. m. mitchellii and C. m. stephensi had ...
Databáze: OpenAIRE