Preliminary Studies of Acute Cadmium Administration Effects on the Calcium-Activated Potassium (SKCa and BKCa) Channels and Na+/K+-ATPase Activity in Isolated Aortic Rings of Rats
| Autor: | Gilson Brás Broseghini-Filho, Ariane Calazans Teixeira, Camila Cruz Pereira Almenara, Dalton Valentim Vassallo, Jhuli Keli Angeli, David Chaves Felicio da Silva, Alessandra Simão Padilha |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Endocrinology Diabetes and Metabolism Clinical Biochemistry chemistry.chemical_element 030204 cardiovascular system & hematology Calcium Apamin Biochemistry Inorganic Chemistry 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Internal medicine medicine Na+/K+-ATPase Cadmium Tetraethylammonium Calcium channel Biochemistry (medical) General Medicine Iberiotoxin Potassium channel 030104 developmental biology Endocrinology chemistry |
| Zdroj: | Biological Trace Element Research. 183:325-334 |
| ISSN: | 1559-0720 0163-4984 |
| Popis: | Cadmium is an environmental pollutant closely linked with cardiovascular diseases that seems to involve endothelium dysfunction and reduced nitric oxide (NO) bioavailability. Knowing that NO causes dilatation through the activation of potassium channels and Na+/K+-ATPase, we aimed to determine whether acute cadmium administration (10 μM) alters the participation of K+ channels, voltage-activated calcium channel, and Na+/K+-ATPase activity in vascular function of isolated aortic rings of rats. Cadmium did not modify the acetylcholine-induced relaxation. After L-NAME addition, the relaxation induced by acetylcholine was abolished in presence or absence of cadmium, suggesting that acutely, this metal did not change NO release. However, tetraethylammonium (a nonselective K+ channels blocker) reduced acetylcholine-induced relaxation but this effect was lower in the preparations with cadmium, suggesting a decrease of K+ channels function in acetylcholine response after cadmium incubation. Apamin (a selective blocker of small Ca2+-activated K+ channels—SKCa), iberiotoxin (a selective blocker of large-conductance Ca2+-activated K+ channels—BKCa), and verapamil (a blocker of calcium channel) reduced the endothelium-dependent relaxation only in the absence of cadmium. Finally, cadmium decreases Na+/K+-ATPase activity. Our results provide evidence that the cadmium acute incubation unaffected the calcium-activated potassium channels (SKCa and BKCa) and voltage-calcium channels on the acetylcholine vasodilatation. In addition, acute cadmium incubation seems to reduce the Na+/K+-ATPase activity. |
| Databáze: | OpenAIRE |
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