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Background & Aim Adipose tissue provides an abundant source of stromal vascular fraction (SVF), which resides a potential regenerative medicine named mesenchymal stem cells (MSC). Storage period and temperature before isolation may impact the quality of isolated cells. Here, we identify the effect of storage periods and temperatures to SVF viability and cell number along with the MSC recovery. Methods, Results & Conclusion A total of 70 ml donor-adipose tissue was divided into two different groups, room temperature (RT) and 4-8°C. SVF was isolated using manual method. Each group was process after stored for 4, 5, 6, 7, and 12 days. After it passed each incubation time, adipose samples were processed enzymatically until SVF was obtained. The viability of SVF stored at RT was decreasing until 40%, but relatively constant when stored at 4-8°C (>75%). Nevertheless, the sample stored at RT revealed to have better proliferation and colonization than sample stored at 4-8°C. The sample retained the proliferation capability when stored at RT up to 7 days, but only up to 5 days when stored at 4-8°C. The average of SVF number was higher in the sample stored at RT (8,552,315 cell/mL) compared to the sample at 4-8°C (4,581,111 cell/mL). The highest SVF number was observed in sample stored at 4 days before isolation. The average of MSC recovery was also higher at RT compared to sample stored at 4-8°C, which was 6% and 3%, respectively. In conclusion, adipose tissue is preferable to be stored at RT before isolation for higher SVF total number and MSC recovery. The adipose tissue should be processed immadiately or stored only up to 4 days after sample collection. |
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