Curcumin induced up-regulation of Myelin basic protein (MBP) ameliorates sodium arsenite induced neurotoxicity in developing rat cerebellum
| Autor: | Pushpa Dhar, Raj D. Mehra, Parul Kaushal |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Antioxidant
Sodium arsenite biology medicine.medical_treatment Neurotoxicity Morris water navigation task Pharmacology medicine.disease Luxol fast blue stain Pathology and Forensic Medicine Myelin basic protein chemistry.chemical_compound Myelin medicine.anatomical_structure chemistry Immunology Curcumin medicine biology.protein Anatomy |
| Zdroj: | Journal of the Anatomical Society of India. 63:3-11 |
| ISSN: | 0003-2778 |
| DOI: | 10.1016/j.jasi.2014.04.001 |
| Popis: | Introduction The aim of the present study is to evaluate the modulatory role of Curcumin on cerebellar Myelin basic protein (MBP) expression and associated behavioral parameters by histochemistry and Morris Water Maze (MWM) following exposure of rat pups to sodium arsenite (NaAsO 2 ). Methods After obtaining the ethical clearance, pregnant Wistar rats were issued from the Central Animal Facility (CAF) and maintained according to the guidelines of Committee for the Purpose of Control and Supervision of Experiments on Animals (CPCSEA). The day of birth of pups was designated as postnatal day zero (PND 0). Mother reared pups were divided into: control (normal, sham) and experimental groups receiving NaAsO 2 alone or NaAsO 2 with Curcumin via intraperitoneal route (i.p.) from PND 1 to 21. Morris Water Maze (MWM) test was carried out on PND 21 to assess the exploratory behavior of the animals. Luxol Fast Blue staining and immuno-localization of MBP was carried out to evaluate the status of myelination in the cerebellar white matter. Results Animals subjected to combined exposure to Curcumin and NaAsO 2 showed improved exploratory behavior besides maintenance of myelin structure and up-regulation of MBP expression in the cerebellar white matter. Discussion The up-regulation of cerebellar MBP expression together with improved exploratory behavior of animals subjected to combined exposure to Curcumin and arsenic (iAs) provides the experimental evidence for mechanistic role of Curcumin in ameliorating NaAsO 2 induced neurotoxicity, probably based on the essential properties of Curcumin such as scavenging free radicals and chelation of arsenic. |
| Databáze: | OpenAIRE |
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