The IN VITRO STUDY OF APOPTOSIS INDUCTION BY L-ASPARAGINASE OF ASPERGILLUS FUMIGATUS (ASPF-6) ON HUMAN LEUKEMIA CELL LINES
| Autor: | Vipina R, Vijaya Chitra |
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| Rok vydání: | 2019 |
| Předmět: | |
| Zdroj: | Asian Journal of Pharmaceutical and Clinical Research. :188-193 |
| ISSN: | 2455-3891 0974-2441 |
| DOI: | 10.22159/ajpcr.2019.v12i7.32158 |
| Popis: | Objective: L-Asparaginase is a relatively widespread enzyme used in the lymphoblastic leukemia chemotherapy. In the present investigation, we report new potential fungal L-asparaginase producer Aspergillus fumigatus from Kadalundi mangrove forest, Kerala. The aim of the present investigation was to evaluate the concentration-dependent cytotoxicity and apoptosis-inducing the effect of extracted enzyme and its ability to induce apoptosis against human leukemia cell lines-HL-60. Methods: The fungal strain was isolated by serial dilution method from rhizosphere soil of mangrove forest. The isolated enzyme purified by 80% ammonium sulfate precipitation, dialysis, and ion exchange chromatography. To determine cytotoxicity and level of apoptosis, three different tests were performed: (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, JC-1 flow cytometry, and Annexin V/propidium iodide (PI) flow cytometric DNA fragmentation. The test compound efficiency compared with positive drug doxorubicin against HL-60 cell lines. Results: The test compound exhibited a concentration-dependent cytotoxic effect on proliferation of HL-60 cell lines (IC-50 12.39 U) with a different level of apoptosis induction (LR-11.3%). Conclusion: A. fumigatus derived L-asparaginase may be clinically useful and results in better utilization for limited fungal-derived drug resources and improve financial feasibility as a therapeutic option for human leukemia. |
| Databáze: | OpenAIRE |
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