Effect of green light on the amount and activity of NDH-1-PSI supercomplex in Synechocystis sp. strain PCC 6803
| Autor: | Fudan Gao, Weimin Ma, Teruo Ogawa |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine Gel electrophoresis Cyanobacteria biology Strain (chemistry) Physiology Chemistry Plant physiology Plant Science biology.organism_classification 01 natural sciences Electron transport chain 03 medical and health sciences 030104 developmental biology Thylakoid Biophysics Linker Chlorophyll fluorescence 010606 plant biology & botany |
| Zdroj: | Photosynthetica. 56:316-321 |
| ISSN: | 1573-9058 0300-3604 |
| Popis: | Cyanobacterial NDH-1 interacts with PSI to form NDH-1-PSI supercomplex. CpcG2, a linker protein for the PSI-specific peripheral antenna CpcG2-phycobilisome, is essential for stabilization of the supercomplex. Green light (GL) increased the expression of CpcG2 but had little effect, if any, on the expression of NDH-1 and PSI, when compared to the abundance of these components under red light (RL). The increased expression of CpcG2 intensified the band of NDH-1-PSI supercomplex after blue-native gel electrophoresis of the thylakoid membrane, possibly by stabilizing the supercomplex. The activity of NDH-1-dependent cyclic electron transport around PSI increased when cells grown under RL were transferred to a low intensity GL but was suppressed when cells were grown under high intensities of GL. The functionality of PSI showed the same trend. We thus conclude that GL increases the expression of CpcG2, thereby increasing the abundance of the NDH-1-PSI supercomplex and its activity at low GL but not at higher GL. |
| Databáze: | OpenAIRE |
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