miR-100-5p Inhibits Malignant Behavior of Chordoma Cells by Targeting IGF1R
| Autor: | Yiyang Yu, Yi Huang, Wei Guo, Xiaodong Tang, Jianfang Niu, Jingbing Lou, Tingting Ren, Xin Liang, Kang Yang, Hongliang Zhang, Wei Wang |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
musculoskeletal diseases
0301 basic medicine medicine.diagnostic_test Chemistry Cell growth Growth factor medicine.medical_treatment medicine.disease Flow cytometry 03 medical and health sciences 030104 developmental biology 0302 clinical medicine Oncology Western blot In vivo 030220 oncology & carcinogenesis microRNA Cancer research medicine Chordoma Insulin-like growth factor 1 receptor |
| Zdroj: | Cancer Management and Research. 12:4129-4137 |
| ISSN: | 1179-1322 |
| DOI: | 10.2147/cmar.s252185 |
| Popis: | Purpose Our research aimed to illuminate the role of miR-100-5p in chordoma and potential mechanism. Materials and methods We used microRNA array analysis to explore differentially expressed miRNAs in chordoma tissue and then verified by qRT-PCR. Cell proliferation and transwell assay were used to evaluate the function of miR-100-5p. Cell apoptosis was analyzed by flow cytometry, and using biological software, we predicted that the insulin-like growth factor 1 receptor (IGF1R) could be the target gene of miR-100-5p, which was then validated by dual luciferase assays and Western blot. Results miR-100-5p was downregulated in chordoma tissues. Overexpression of miR-100-5p could suppress the growth of chordoma both in vitro and in vivo, and miR-100-5p could inhibit the migration and invasion of chordoma cells partially by suppressing epithelial-mesenchymal transition (EMT). Furthermore, IGF1R was validated as the target gene of miR-100-5p and expressed in most chordoma tissues. Conclusion In conclusion, our results showed that miR-100-5p was lowly expressed in chordoma and inhibited tumor malignant progression by targeting IGF1R. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|