Pathogenicity evaluation of Ralstonia pseudosolanacearum race 4 on ginger by leaf-clipping inoculation
Autor: | Kazutaka Yano, Momoka Imamura, Kazuhiro Iiyama, Mitsuo Horita, Htet Wai Wai Kyaw, Kenichi Tsuchiya, Naruto Furuya, Tomomi Inoue |
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Rok vydání: | 2021 |
Předmět: | |
Zdroj: | Journal of General Plant Pathology. 87:269-272 |
ISSN: | 1610-739X 1345-2630 |
DOI: | 10.1007/s10327-021-01017-6 |
Popis: | An easy, rapid method has been needed to test the pathogenicity of strains of Ralstonia pseudosolanacearum, the cause of serious bacterial wilt on ginger (Zingiber officinale). When ginger leaves were clip-inoculated with race 4 strains, yellowing and V-shaped lesions developed on leaves, but no lesions formed after inoculation with race 1 strain or on mock-inoculated leaves. Tests of different bacterial concentrations as inoculum showed that the optimal concentration was greater than or equal to 106 cfu/ml. This simple, high-throughput leaf clipping inoculation method provides an efficient, easy method to evaluate pathogenicity of R. pseudosolanacearum race 4 on ginger. |
Databáze: | OpenAIRE |
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