Kinetics of growth and antibody production by a hybridoma cell line in a perfusion culture

Autor:	T. Craig Seamans, Wei Shou Hu
Rok vydání:	1990
Předmět:	Antibody production Hybridoma cell Perfusion Culture Perfusion rate Biochemistry Chemistry Microfiltration membrane Kinetics Continuous perfusion Biophysics Applied Microbiology and Biotechnology Biotechnology
Zdroj:	Journal of Fermentation and Bioengineering. 70:241-245
ISSN:	0922-338X
DOI:	10.1016/0922-338x(90)90056-3
Popis:	A hybridoma cell line, AFP-27, was cultivated in a stirred tank with continuous perfusion. Microfiltration membrane was used to partially retain the cells. Using off-line measurement of glucose concentration the perfusion rate was increased in steps with increasing cell concentration. The specific rates of growth, glucose consumption, and antibody production appear to decrease with time. The need for kinetic models for optimization is discussed.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::c3bd7cda5a93c646972f124b811f5d85 https://doi.org/10.1016/0922-338x(90)90056-3 Zobrazit plný text záznamu