Characterization ofEntamoeba histolytica-induced dephosphorylation in Jurkat cells
| Autor: | J. E. Teixeira, B. J. Mann |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Phosphatase
Lectin General Medicine Biology biology.organism_classification Molecular biology Jurkat cells General Biochemistry Genetics and Molecular Biology Cell biology Dephosphorylation Entamoeba histolytica Apoptosis parasitic diseases biology.protein Phosphorylation Tyrosine General Agricultural and Biological Sciences |
| Zdroj: | Journal of Biosciences. 27:615-618 |
| ISSN: | 0973-7138 0250-5991 |
| DOI: | 10.1007/bf02704856 |
| Popis: | Entamoeba histolytica killing of host cells is contact dependent and mediated by a Gal/GalNAc lectin. Upon contact with amoeba a rapid and extensive dephosphorylation of tyrosine phosphorylated host cell proteins is observed. This effect is mediated by the Gal/GalNAc lectin. However, it requires intact cells, as purified lectin failed to induce dephosphorylation in Jurkat cells. The nonpathogenic, but morphologically identical amoeba,Entamoeba moshkovskii also did not induce dephosphorylation in target cells. Treatment of Jurkat cells with phosphotyrosine phosphatase inhibitors has shown that a host phosphatase is responsible for dephosphorylation. However, it was found that the CD45 phosphotase was not necessary for dephosphorylation of host cell proteins. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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