The early stage of polyphosphate accumulation in saccharomyces cerevisiae: comparative study by extraction and DAPI staining
| Autor: | Valerii Yashin, Nadeschda Andreeva, Airat Valiakhmetov, Tatiana Kulakovskaya, L. P. Ryazanova, Igor S. Kulaev, V. M. Vagabov |
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| Rok vydání: | 2011 |
| Předmět: |
Strain (chemistry)
Saccharomyces cerevisiae General Medicine Vacuole Biology biology.organism_classification digestive system diseases Yeast Staining chemistry.chemical_compound surgical procedures operative chemistry Biochemistry Cytoplasm otorhinolaryngologic diseases DAPI neoplasms Exopolyphosphatase |
| Zdroj: | Advances in Bioscience and Biotechnology. :293-297 |
| ISSN: | 2156-8502 2156-8456 |
| Popis: | Inorganic polyphosphate (PolyP), a bioactive polymer with multiple functions, plays a key role in biomineralizaion and phosphorus homeostasis in yeasts. After phosphate starvation, the cells of Saccharomyces cerevisiae restored their pool of PolyP during the first 30 min of incubation in the media containing phosphate and carbon sources. The cells of parent strain accumulated PolyP both in glucose and ethanol-containing media. In the medium with glucose, the strain with inactivated PPX1 and PPN1 genes (encoding two major yeast polyphosphatases) accumulated 2-fold more PolyP than the parent strain. The PolyP in the mutant cells had a greater average chain length compared to the parent strain. The strain with inactivated exopolyphosphatase genes РРХ1 and PPN1 was incapable of PolyP synthesis in the medium with ethanol. The in vivo staining of cells with DAPI show that in the cells of parent strain PolyP appeared first in cytoplasm and mitochondria under cultivation in glucose-containing medium and in cytoplasm and vacuoles in the medium with ethanol. In the ΔPPX1ΔPPN1double mutant PolyP accumulated in cytoplasm and vacuoles under cultivation in glucose-contained medium. |
| Databáze: | OpenAIRE |
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