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Aim - to analyze the frequency of somatic mutations in the KRAS gene in tumor samples of Southern Russia patients with colorectal cancer, and also to assess the potential relationship between KRAS mutations and the risk of metastatic development in patients. The procedure for DNA extraction from paraffin-embedded tissue blocks (FFPE) included standard dewaxing of sections in or-thoxylene. From the presence of sections of registration of total DNA preparations. Using the Real-Time-PCR-KRAS-7M reagent kit (Biolink, Russia), 7 SNP mutations (Single Nucleotide Polymor-phism) were detected in the 12 and 13 codons of the KRAS gene: G12C, G12S, G12R, G12V, G12D , G12A, G13D using the Bio-Rad CFX96 thermal cycler (Bio-Rad, USA). All statistical analyzes were car-ried out in the package IBM SPSS Statistics v. 23.0, Microsoft Excel 2013 and STATISTICA 8.0. A statistically significant association with metastasis was observed in the presence / absence of G12D in the KRAS gene, namely, the tendency to decrease the risk of metastasis (OR = 0.625, 95 % CI 0.389–1,006, p = 0.053). Progression of colorectal cancer is significantly associated with an increase in the stage of the disease (OR = 2.061, 95 % CI 1.790–2.373, p |
