Hepatoprotective and in Vivo Antioxidant Enzyme Activity of Phenol Rich Leaf Extract of Amaranthus spinosus

Autor: Ndu-Osuji Ijeoma, Nnaoma I. E, Ahamefula Chisom O, Joel Obinna
Rok vydání: 2022
Předmět:
Zdroj: Scholars Journal of Applied Medical Sciences. 10:983-990
ISSN: 2320-6691
2347-954X
DOI: 10.36347/sjams.2022.v10i06.018
Popis: This study was designed to evaluate the total phenol content, in vivo and antioxidant activity and hepatoprotective activity of methanol leaf extract of Amaranthus spinosus. 15 wistar albino rats having an average mean weight of 104.64 kg was divided into five groups consisting of three animals each group. Group A served as normal control, group B served as negative control, group C served as positive control while group D and E served as test control administered with different doses of Amaranthus spinosus. Hepatotoxicity was induced using CCL4 injected interperitoneally for groups 2-5. The experimental period lasted for seven days after which the animals were sacrificed and their blood collected for analysis. The phenol content was estimated using Folin-Ciocalteu reagent method. The in-vivo antioxidant screening as also estimated determining the concentration in serum of SOD, CAT and MDA and the liver function test was evaluated by also determining the concentration in serum of ALT, AST and ALP spectrophotometrically. The results obtained were presented as mean ± SEM. There was significant increase in the SOD activities of treated groups compared to the negative control group. Result showed a significant (p
Databáze: OpenAIRE