Development of species-specific marker and molecular diversity of Conogethes punctiferalis and Conogethes sahyadriensis (Lepidoptera: Crambidae)
| Autor: | R.K. Chandel, D. Sagar, A.K. Chakravarthy, P. R. Shashank, V. Kammar |
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| Rok vydání: | 2021 |
| Předmět: | |
| Zdroj: | Journal of Environmental Biology. 42:271-279 |
| ISSN: | 2394-0379 0254-8704 |
| DOI: | 10.22438/jeb/42/2/mrn-1322 |
| Popis: | Aim: The aim of this study was to develop species specific marker for quick identification of two Conogethes species and to study their molecular diversity. Methodology: Species-specific markers were developed using mitochondrial cytochrome oxidase 1 (COI) partial gene sequences of Conogethes punctiferalis and C. sahyadriensis and they were validated. Phylogenetic tree was constructed using MEGA X program, tree robustness was evaluated by bootstrapping with 1000 replicates with all sequences of C. punctiferalis and C. sahyadriensis. Results: Bioinformatic analysis of C. punctiferalis and C. sahyadriensis COI sequences revealed that they have sufficient genetic variability to develop species specific marker. The COI based species-specific markers amplified an expected fragment size of 333 bp for C. punctiferalis and 522 bp for C. sahyadriensis, which clearly differentiate these two species. Interpretation: The species specific marker developed in this study will be useful in quick identification of C. punctiferalis and C. sahyadriensis. The use of COI gene as a marker can provide a better estimate of genetic diversity, as it is maternally inherited and can be helpful to understand evolutionary processes. Key words: Conogethes punctiferalis, C. sahyadriensis, MtCOI, Molecular diversity, Species-specific marker |
| Databáze: | OpenAIRE |
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