Evaluation of Community-Level Physiological Profiling for Monitoring Microbial Community Function in Aquaculture Ponds

Autor: Gerald L. Kurten, Aaron Barkoh
Rok vydání: 2015
Předmět:
Zdroj: North American Journal of Aquaculture. 78:34-44
ISSN: 1548-8454
1522-2055
DOI: 10.1080/15222055.2015.1079580
Popis: Microbial communities in aquaculture ponds play pivotal roles in pond productivity and fish production success. Nonetheless, culturists do not consider this variable in pond management decision making likely because of a lack of practical assessment tools. Therefore, we evaluated the community-level physiological profiling (CLPP) technique for characterizing microbial community function for use in aquaculture. Unfiltered water samples collected from various plastic-lined ponds over time were incubated on Biolog EcoPlates. Responses (substrate optical densities) were measured at 24-h intervals for up to 12 d to determine the best incubation interval and the number of required replicates needed for determining microbial community function in aquaculture ponds. The repeatability and within-pond variability of the CLPP metrics were also evaluated. During cooler months and in ponds with no fish and low apparent microbial activity, a 96-h incubation period was required to differentiate microbial communi...
Databáze: OpenAIRE
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