Rapid Identification of KL49 Acinetobacter baumannii Associated with Clinical Mortality
| Autor: | Huaisheng Chen, Weiyuan Wu, Tianle Zhang, Qiuyang Deng, Quanming Zou, Xueyan Liu, Xiyao Yang, Zhou Liu, Wei Huang, Chuchu Lin, Yuxin Zhong, Jinyong Zhang, Yutian Luo, Ruiqin Cui, Min Zhang, Hao Zeng, Yuemei Lu, Shiyi Liu, Yun Shi, Jinsong Wu |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Pharmacology Whole genome sequencing biology 030106 microbiology Locus (genetics) biology.organism_classification Phenotype Acinetobacter baumannii Microbiology law.invention Rapid identification 03 medical and health sciences 0302 clinical medicine Infectious Diseases Phylogenetics law Pharmacology (medical) 030212 general & internal medicine Gene Polymerase chain reaction |
| Zdroj: | Infection and Drug Resistance. 13:4125-4132 |
| ISSN: | 1178-6973 |
| DOI: | 10.2147/idr.s278891 |
| Popis: | Objective We aimed to establish a tool for rapid identification of KL49 Acinetobacter baumannii. Methods Based on the capsular polysaccharide (CPS) synthesis genes database, we investigated the distribution of K locus type 49 (KL49) genes in other KL types and established a rapid identification method for KL49. We collected 61 clinical carbapenem-resistant A. baumannii (CRAB) strains, identified KL49 by gtr100 detection, and used whole genome sequencing (WGS) for verification. A mouse pneumonia model was used to confirm the hypervirulence phenotype. We tested the presence of gtr100 gene in 165 CRAB strains from three provinces in China and evaluated the correlation of gtr100 carrying CRAB infection with mortality. Results The gtr100 gene is the CPS synthesis gene found only in KL49. We screened out nine WGS-validated KL49 strains from 61 CRAB clinical strains using polymerase chain reaction (PCR) to detect the gtr100 gene. The survival rates of KL49 strains were significantly lower than nonKL49 strains in a mouse pneumonia model. The survival rates of LAC-4 gtr100 knockout strain decreased significantly. Analysis of phylogenetics showed the worldwide spread of KL49 A. baumannii. Infection of gtr100 carrying CRAB is an independent risk for mortality (OR, 10.76; 95%CI: 3.08-37.55; p |
| Databáze: | OpenAIRE |
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