Electrophysiological Investigation of NMDA Current Properties in Brain Slices
| Autor: | Natalia Lozovaya, Alexandre Pons-Bennaceur |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Postsynaptic Current Chemistry musculoskeletal neural and ocular physiology Glutamate receptor AMPA receptor Neurotransmission Synapse 03 medical and health sciences Electrophysiology 030104 developmental biology 0302 clinical medicine nervous system Excitatory postsynaptic potential NMDA receptor Neuroscience 030217 neurology & neurosurgery |
| Zdroj: | Methods in Molecular Biology ISBN: 9781493973200 |
| DOI: | 10.1007/978-1-4939-7321-7_12 |
| Popis: | Analysis of electrophysiological properties of NMDARs and NMDAR-mediated synaptic transmission in identified neurons and synapses in brain slices is a major step in understanding their function in normal and pathological neuronal brain networks. In many central synapses excitatory postsynaptic currents (EPSCs) are mediated by excitatory neurotransmitter glutamate that activates colocalized AMPAR and NMDAR generating a complex EPSC. Here, we describe the methods commonly used in brain slices to study the electrophysiological properties of NMDAR-mediated component of spontaneous or evoked EPSCs by extracellular stimulation or by stimulating synaptically connected neurons. This approach is based on whole-cell patch-clamp recordings, pharmacological tools, and the analysis of the difference in temporal parameters between the AMPA and NMDA receptors. It allows pinpointing of the basic functional properties of NMDARs that are specific to identified brain regions, neurons, and synapses of wild-type or genetically manipulated mice. |
| Databáze: | OpenAIRE |
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