Gene Transformation by Chitosan Nanoparticle to Enhance Fatty Acid Production in Zea mays (L.)

Autor:	Aya Tarek Rizk, Rawan Adel Abd-Elwahab, Nada Ahmed Kamal, Kareem Mohammed Abd-Elatty, Islam Hamdy El-Shenawy, Mohammed Ibrahim Dahab, Eman Tawfik Hussien, Heba Mahmoud Farouk
Rok vydání:	2021
Předmět:	chemistry.chemical_classification Chitosan chemistry.chemical_compound chemistry Biochemistry Genetics Fatty acid Nanoparticle Agronomy and Crop Science Applied Microbiology and Biotechnology Zea mays Biotechnology
Zdroj:	Romanian Biotechnological Letters. 26:2971-2978
ISSN:	2248-3942 1224-5984
DOI:	10.25083/rbl/26.5/2971.2978
Popis:	Zea mays is an important crop and an essential source of fatty acids. Hence, increasing and adding new fatty acids led to the enhancement of these properties. Transformation of external Acetyl-CoA gene (Aco) can enhance fatty acid components, as ACo is expressed into Acetyl-CoA carboxylase (ACCase) enzyme, which is the first essential step in the fatty acid production process. Chitosan nanoparticles are safe and fast polymer nanoparticles that are applied for gene transformation. Conventional PCR was performed for the detection of the ACo gene in both transgenic and nontransgenic maize lines. The results confirm the presence of the gene in the transgenic lines and absence in non-transgenic lines. The Gas chromatography-mass spectrometry (GC-MS) analysis for fatty acid contents in transgenic and non-transgenic maize lines showed an increase in fatty acid contents in transgenic lines compared to non-transgenic ones. Besides, the transgenic maize’s lines produced extra new fatty acids not found in non-transgenic ones.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::c33d170d81dc26fa5edf4d8897e89aaf https://doi.org/10.25083/rbl/26.5/2971.2978 Zobrazit plný text záznamu