The study of DJ-1 protein in tissue specimens, cultured cells and serum of prostate cancer patients

Autor:	L. S. Eremina, I. Yu. Toropygin, V. E. Okhrits, M. A. Kovalyova, K. V. Lisitskaya, A. V. Ivanov, L. I. Kovalyov, Shishkin Ss
Rok vydání:	2011
Předmět:	Gene isoform PCA3 Pathology medicine.medical_specialty business.industry Clinical Biochemistry Hyperplasia urologic and male genital diseases medicine.disease Biochemistry Prostate cancer Cell culture Two dimensional electrophoresis LNCaP medicine Molecular Medicine Biomarker (medicine) business
Zdroj:	Biochemistry (Moscow) Supplement Series B: Biomedical Chemistry. 5:22-28
ISSN:	1990-7516 1990-7508
DOI:	10.1134/s1990750811010070
Popis:	Two electrophoretically distinguishable isoforms of Dj-1 protein have been identified in a proteomic study of tissue specimens obtained from patients with confirmed prostate cancer (PCa) and benign prostatic hyperplasia (BPH). Dj-1 was also found in the cell lines PC-3, DU-145, LNCaP, BPH-1, and the lowest level of Dj-1 was found in BPH-1. An immunochemical study (ELISA) of serum levels of Dj-1, Bcl-2, IGF-1 and IGFBP-3 proteins revealed statistically significant differences between these two groups of patients only for Dj-1 (p = 0.004, the Wilcoxon-Mann-Whitney test). These data suggest that Dj-1 protein is a perspective candidate biomarker for PCa.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::c32d8032a76e5a66c5edbbaf2a64b2e3 https://doi.org/10.1134/s1990750811010070 Zobrazit plný text záznamu Full text from SpringerLink