Identification of phosphocysteine by electrospray mass spectrometry combined with Edman degradation
| Autor: | Christiane Weigt, Rembert Pogge von Strandmann, Helmut E. Meyer, Horst Korte, Wolfgang Hengstenberg |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
chemistry.chemical_classification
Chromatography biology Edman degradation Ethanethiol Organic Chemistry Chemical modification Peptide General Medicine PEP group translocation Protein tyrosine phosphatase biology.organism_classification Biochemistry Analytical Chemistry chemistry.chemical_compound chemistry Phosphoserine Staphylococcus carnosus |
| Zdroj: | Journal of Chromatography A. 712:141-147 |
| ISSN: | 0021-9673 |
| DOI: | 10.1016/0021-9673(95)00457-x |
| Popis: | Phosphocysteine is an intermediate in the phosphoenolpyruvate-dependent phosphotransferase system (PTS) and in the dephosphorylation of phosphotyrosine residues by protein tyrosine phosphatases. A method is described for the direct and non-radioactive localization of phosphocysteine. The phosphorylated Glu-C peptide of the EIIMtl protein of the PTS of Staphylococcus carnosus was identified by LC-electrospray MS and isolated for further analysis. Following chemical modification with alkaline ethanethiol, S-ethylcysteine was identified during Edman degradation, demonstrating that phosphocysteine reacts like phosphoserine. As a control, the unphosphorylated cysteine could not be modified by the alkaline ethanethiol. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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