The complete nucleotide sequence and genome organization of pea streak virus (genus Carlavirus)

Autor:	Mike Bernardy, Zhengnan Li, Yu Xiang, Li Su, Paul A. Wiersma, Zhihui Cheng
Rok vydání:	2015
Předmět:	Genetics Whole genome sequencing biology cDNA library Nucleic acid sequence General Medicine biology.organism_classification Virology Molecular biology Genome Pea streak virus Rapid amplification of cDNA ends ORFS Genomic organization
Zdroj:	Archives of Virology. 160:2651-2654
ISSN:	1432-8798 0304-8608
DOI:	10.1007/s00705-015-2467-2
Popis:	Pea streak virus (PeSV) is a member of the genus Carlavirus in the family Betaflexiviridae. Here, the first complete genome sequence of PeSV was determined by deep sequencing of a cDNA library constructed from dsRNA extracted from a PeSV-infected sample and Rapid Amplification of cDNA Ends (RACE) PCR. The PeSV genome consists of 8041 nucleotides excluding the poly(A) tail and contains six open reading frames (ORFs). The putative peptide encoded by the PeSV ORF6 has an estimated molecular mass of 6.6 kDa and shows no similarity to any known proteins. This differs from typical carlaviruses, whose ORF6 encodes a 12- to 18-kDa cysteine-rich nucleic-acid-binding protein.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::c30e646810036d48ad75c677f7637f2e https://doi.org/10.1007/s00705-015-2467-2 Zobrazit plný text záznamu Full text from SpringerLink