Transposition of IS 1397 in the Family Enterobacteriaceae and First Characterization of ISKpn 1 , a New Insertion Sequence Associated with Klebsiella pneumoniae Palindromic Units

Autor:	Maurice Hofnung, Sophie Bachellier, Jean-Marie Clément, Caroline Wilde
Rok vydání:	2001
Předmět:	Genetics biology Klebsiella pneumoniae Palindrome Klebsiella oxytoca biology.organism_classification medicine.disease_cause Microbiology Enterobacteriaceae Intergenic region Salmonella enterica medicine Insertion sequence Molecular Biology Escherichia coli
Zdroj:	Journal of Bacteriology. 183:4395-4404
ISSN:	1098-5530 0021-9193
Popis:	IS 1397 and ISKpn 1 are IS 3 family members which are specifically inserted into the loop of palindromic units (PUs). IS 1397 is shown to transpose into PUs with sequences close or identical to the Escherichia coli consensus, even in other enterobacteria ( Salmonella enterica serovar Typhimurium, Klebsiella pneumoniae , and Klebsiella oxytoca ). Moreover, we show that homologous intergenic regions containing PUs constitute IS 1397 transpositional hot spots, despite bacterial interspersed mosaic element structures that differ among the three species. ISKpn 1 , described here for the first time, is specific for PUs from K. pneumoniae , in which we discovered it. A sequence comparison between the two insertion sequences allowed us to define a motif possibly accounting for their specificity.
Databáze:	OpenAIRE
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