Analysis of an immunoadjuvant saponin fraction from Quillaja brasiliensis leaves by electrospray ionization ion trap multiple-stage mass spectrometry
| Autor: | Cristina Olivaro, Fernando Ferreira, Zohra Bennadji, Federico Wallace |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
chemistry.chemical_classification Chromatography biology Chemistry Electrospray ionization Saponin Plant Science Oligosaccharide biology.organism_classification Mass spectrometry Biochemistry Ion 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology Aglycone Quillaja Ion trap Agronomy and Crop Science Biotechnology |
| Zdroj: | Phytochemistry Letters. 20:228-233 |
| ISSN: | 1874-3900 |
| DOI: | 10.1016/j.phytol.2017.04.020 |
| Popis: | An immunoadjuvant saponin fraction from Quillaja brasiliensis leaves was investigated by direct infusion and liquid chromatography/electrospray ionization ion trap multiple-stage mass spectrometry in negative ion mode (DI-ESI-IT-MSn and LC-ESI-IT-MSn). The aglycone and the sequence of the oligosaccharide residues at C-3 and C-28 were characterized based on MS2 and MS3 experiments of the [M H]− ions. According to their [M H]− ions, characteristic product ions and retention times, 27 bidesmosidic saponins, bearing four types of triterpenic aglycones, were tentatively identified. |
| Databáze: | OpenAIRE |
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