| Autor: |
Joseph E. Marturano, Catherine K. Kuo, Jeffrey J. Thibodeau, Nathan R. Schiele, Laura A. Hockaday, Friedrich von Flotow, Zachary L. Tochka |
| Rok vydání: |
2015 |
| Předmět: |
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| Zdroj: |
Journal of Orthopaedic Research. 33:874-881 |
| ISSN: |
0736-0266 |
| DOI: |
10.1002/jor.22880 |
| Popis: |
Tendon injuries are common and heal poorly. Strategies to regenerate or replace injured tendons are challenged by an incomplete understanding of normal tendon development. Our previous study showed that embryonic tendon elastic modulus increases as a function of developmental stage. Inhibition of enzymatic collagen crosslink formation abrogated increases in tendon elastic modulus at late developmental stages, but did not affect increases in elastic modulus of early stage embryonic tendons. Here, we aimed to identify potential contributors to the mechanical properties of these early stage embryonic tendons. We characterized tendon progenitor cells in early stage embryonic tendons, and the influence of actin cytoskeleton disruption on tissue elastic modulus. Cells were closely packed in embryonic tendons, and did not change in density during early development. We observed an organized network of actin filaments that seemed contiguous between adjacent cells. The actin filaments exhibited a crimp pattern with a period and amplitude that matched the crimp of collagen fibers at each developmental stage. Chemical disruption of the actin cytoskeleton decreased tendon tissue elastic modulus, measured by atomic force microscopy. Our results demonstrate that early developmental stage embryonic tendons possess a well organized actin cytoskeleton network that contributes significantly to tendon tissue mechanical properties. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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