Physicochemical Characterization of Protein Hydrolysates Produced by Autolysis of Jumbo Squid (Dosidicus gigas) Byproducts
| Autor: | Hisila Santacruz-Ortega, Joe Luis Arias-Moscoso, Wilfrido Torres-Arreola, Ofelia Rouzaud-Sández, Josafat Marina Ezquerra-Brauer, Amir Darío Maldonado-Arce, Enrique Márquez-Ríos, Martha Gabriela Gaxiola-Cortés |
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| Rok vydání: | 2014 |
| Předmět: |
chemistry.chemical_classification
Autolysis (biology) Chromatography medicine.diagnostic_test Chemistry Proteolysis Biophysics Bioengineering Peptide Applied Microbiology and Biotechnology Hydrolysate Analytical Chemistry Amino acid NMR spectra database Hydrolysis medicine Particle size Food Science |
| Zdroj: | Food Biophysics. 10:145-154 |
| ISSN: | 1557-1866 1557-1858 |
| DOI: | 10.1007/s11483-014-9374-z |
| Popis: | The physicochemical characteristics of protein hydrolysates of jumbo squid (Dosidicus gigas) byproducts (JSBP) produced by endogenous proteases at two different pH values (5.0 and 7.0) were studied using destructive and nondestructive methods. Reaction mixture samples were collected at different interval times during hydrolysis to monitor changes in the level of hydrolysis (DH) using the OPA method, the protein peptide molecular masses (MM), and SDS-PAGE. The DH increased from 3.5 to 11.2 % at pH 5.0 and from 4.8 to 17.5 % at pH 7.0. Both pH treatments exhibited similar degradation patterns with progressive proteolysis and, after 120 min of hydrolysis, yielded hydrolysates that contained MM |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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