| Autor: |
van der Kammen Ra, Evers Ee, ten Klooster Jp, John G. Collard |
| Rok vydání: |
2000 |
| Předmět: |
|
| DOI: |
10.1016/s0076-6879(00)25461-x |
| Popis: |
Publisher Summary This chapter discusses Rho-like guanosine 5'-triphosphates (GTPases) in tumor cell invasion. Metastasizing tumor cells are characterized by their invasion into surrounding tissues. After entering the bloodstream, the malignant tumor cells extravasate at distant organs to establish metastases. In search for genes that can induce invasion and metastasis of T-lymphoma cells, the chapter identifies the Tiaml (T-lymphoma invasion and metastasis 1) gene in a retroviral insertional mutagenesis screen by selecting for invasive variants of the parental, noninvasive BW5147 T-lymphoma cell line. The functions of Rho-like GTPase in epithelial cell–cell adhesion and migration is also discussed in the chapter. The chapter discusses the fibroblast invasion assay and concludes with a discussion of the transwell migration assay. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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