Comparison of Different Methods for Drug Susceptibility Testing of Mycobacterium tuberculosis to Rifampicin

Autor:	T. V. Umpeleva, E. A. Mazurina, D. V. Vakhrusheva, N. I. Eremeeva
Rok vydání:	2022
Předmět:	genetic structures General Medicine bacterial infections and mycoses
Zdroj:	Tuberculosis and Lung Diseases. 100:41-48
ISSN:	2542-1506 2075-1230
Popis:	The objective: to compare results of drug susceptibility testing to rifampicin by molecular genetic methods and phenotypic tests of Mycobacterium tuberculosis isolates obtained from clinical specimens of tuberculosis patients.Subjects and Methods. 915 samples of M. tuberculosis DNA and 426 cultures were used in this study. Genotypic tests (TB-TEST (BIOCIP-IMB, Russia), GenoType MTBDRplusV2) and phenotypic technologies (absolute concentration method, Bactec MGIT 960 system, Sensititre Myco TB kit) were used.Results. A high percentage (98.7%; CI 97.7-99.7%) of confirmation of the results of the molecular genetic test (TB-TEST) by the phenotypic test (absolute concentration method) was demonstrated. In some cases, the Bactec MGIT 960 system as well as the absolute concentration method were shown to produce false negative results of rifampicin resistance in some cases.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::c2aa14402c2caefcb898475f31f45a03 https://doi.org/10.21292/2075-1230-2022-100-1-41-48 Zobrazit plný text záznamu