| Popis: |
Background Lung adenocarcinoma (LUAD) is one of the most prevalent and lethal malignancies worldwide. Synaptotagmins (SYTs) are a group of transmembrane proteins of which dysregulation has been reported in various cancers. Here, we analyzed the role of SYT2/11/13/15 in LUAD using bioinformatic methods.Methods In TCGA-LUAD cohort, we compared the expression level of SYTs in LUAD and normal tissue. To determine their biological functions, protein-protein interaction (PPI) network was constructed and GO/KEGG analysis were performed. GSEA were used to find the pathway changes. DNA methylation changes in SYT2/11/13/15 were investigated based on MethSurv. The impact on immune cell infiltration were revealed using TIMER database. The diagnostic power of SYT2/11/13/15 were studied using Kaplan-Meier plotter, diagnostic receiver operating characteristic (ROC) curves, nomogram model, and Cox regression analysis.Results In brief, we found that SYT2/13 were upregulated and SYT11/15 were down-regulated in LUAD, high expression of SYT2/11/15 predicted a favorable prognosis while SYT13 indicated a poor one. Functional analysis showed that SYTs mainly involved in neuroactive signal transduction. Several CpG islands of SYTs genes correlated to poor prognosis were identified. Cox regression analysis showed that SYT2/11/13/15 are independent risk factors for overall survival (OS) and disease-specific survival (DSS) of LUAD patients. SYT11/15 were closely related to immune infiltration in LUAD, SYT11 correlated to CD8 + T cell, dendrite cell (DC), macrophages. SYT15 correlated to CD4 + T cell, DC, and myoid derived suppressor cell (MDSC).Conclusion To sum up, we found that SYT2/11/13/15 were differentially expressed and were potential prognostic biomarkers, and may modulate immune infiltration in LUAD. |
