Three Modifications of the Dodecyl Sulfate Method of Kayet al.for Isolating DNA from Calf Thymus

Autor:	David Volkman, Alexander L. Dounce, Richard Turk, Regina Ickowicz
Rok vydání:	1973
Předmět:	chemistry.chemical_compound Chromatography Dodecyl sulfate Biochemistry medicine.diagnostic_test Chemistry Proteolysis Genetics medicine DNA
Zdroj:	Preparative Biochemistry. 3:409-423
ISSN:	0032-7484
DOI:	10.1080/00327487308061526
Popis:	Two rapid methods for the isolation of calf thymus DNA are described which are modifications of the method of Kay et al. 1 for isolating DNA. The principal changes introduced are that degelation of the DNA-nucleo-protein gels is accomplished rapidly by the use of mild shear or by pH adjustment in the presence of isopropanol instead of by the more time-consuming autolytic proteolysis used in the original method.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::c2a496f9c6c3c46463295448c0d51e15 https://doi.org/10.1080/00327487308061526 Zobrazit plný text záznamu