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The culture of plant cell, issues and organs under, defined laborato, onditions both pu, ndapplied applications. A unique feature of plant cells is that they exhibit totipotency. This enables intact, fertile plants to be regenerated from tissues which are given the appropriate stimuli from exogenously supplied growth regulators, such as auxins and cytokinins, in the culture medium. The ability to regenerate plants is fundamental to the multiplication of elite individuals by micropropagation. Additionally, the genetic engineering of plants, through exposure of somaclonal variation, somatic hybridization by protoplast fusion and transformation involving Agrobacterium-mediated gene delivery or direct DNA uptake, also neccessitates reproducible plant regeneration. |
