THE ANTIBODY RESPONSE TO GRAM NEGATIVE ORGANISMS: AN EXPLANATION OF THE DIFFERENCES BETWEEN BACTERIAL AND HEMAGGLUTINATING ANTIBODY TITERS
| Autor: | Muriel Plonko, Edward F. Rabe |
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| Rok vydání: | 1954 |
| Předmět: | |
| Zdroj: | Pediatrics. 14:351-356 |
| ISSN: | 1098-4275 0031-4005 |
| DOI: | 10.1542/peds.14.4.351 |
| Popis: | 1. 0 antigens to one strain each of Klebsiella type 55-97, Paracolon 0, Group 3, and S. Choleraesuis produce antibodies in rabbits which can be measured by the following techniques: bacterial agglutination, indirect hemagglutination using red blood cells sensitized with bacterial suspension, bacterial polysaccharide or Seitz filtrate of broth culture of the bacteria, and the hemolytic modificaion of the hemagglutination test using bacterial suspension or polysaccharide sensitized red blood cells as antigen. 2. The highest absolute titers are recorded with bacterial suspension sensitized red blood cells in either the hemagglutination test or the hemolytic modification of this test. Titer differences between paired sera are measured as well by the bacterial agglutination test as by the indirect hemaglutination test. This is true for any of the three bacterial antigens studies. 3. Cross absorption tests with a Klebsiella antiserum and four forms of the homologous antigen indicate that the Klebsiella organism least two antigenic components giving rise to distinct antibodies. 4. Differences in titer between bacterial and hemagglutinating antibodies for Klebsiella are the result of the existence of two distinct antigenic components in the bacteria which are measured separately by each test. |
| Databáze: | OpenAIRE |
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