| Popis: |
Smokeless tobacco (ST) is commonly used by black South African women because of its perceived medicina lproperties. This pilot study sought to evaluate the antibacterial effect of the local ST products on salivary microflora, using a growth medium. The five ST products tested included three traditional mixtures (brands A, B, C) and two popular industrially manufactured brands (Taxi and Ntsu). The following dilutions of supernatant solution of aqueous extracts of ST were prepared: ‘pure’ extract (100%), 75%, 50% and 25%. 50 μl of each ST extract of varying dilution was aseptically placed on sterilised grade 1 filter paper discs of diameterisc whichwasthenplacedontLsalivasuspensioninbloodagarplLs (BAPs) and incubated at 37°C aerobically and anaerobically for 72 hours. All BAPs, in duplicate, were then visually examined for inhibition zones after 24-, 48- and 72 hours. No bacteria linhibition zone was observed under aerobic oranaerobic conditions with any of the ST products tested, independent of tim... |
