| Autor: |
Renzhong Luo, Qingxiang Zeng, Wenlong Liu, Yan Li, Shengbao Yan, Yiquan Tang, Lifeng Zhou |
| Rok vydání: |
2019 |
| Předmět: |
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| Zdroj: |
SSRN Electronic Journal. |
| ISSN: |
1556-5068 |
| Popis: |
Background: IL-35 and IL-35 induced IL-35-producing regulatory T cells (iTr35) have been reported to inhibit Th2 response in allergic rhinitis (AR). However, the effect of IL-35 and iTr35 on type II innate lymphoid cells (ILC2) in AR is not well characterized. Objective: We aimed to explore the effect of IL-35 and iTr35 on type II innate lymphoid cells (ILC2) in AR. Methods: Twenty-five AR patients and 20 healthy controls were recruited. The proteint expression of IL-35, the proportion of ILC2, IL-4+ILC2, IL-5+ILC2, IL-13+ILC2 and IL-35+CD4+CD25+ cells were detected by enzyme-linked immunosorbnent assay (ELISA) and flow cytometry. The expression and regulation of IL-35 receptor in ILC2 were analyzed by real-time PCR. The effect of IL-35 on ILC2 differentiation and cytokine production were analyzed by real-time PCR and ELISA. The iTr35 were cocultured with ILC2 in Transwell or non-Transwell system to explore the regulation of the iTr35 on ILC2 differentiation and function using RT-PCR and ELISA. AR mice models were also established to confirm the role of IL-35 in the regulation of ILC2 in vivo. Results: AR patients had decreased IL-35 expression and iTr35 proportion and increased proportion of ILC2 and type II cytokines compared with controls (P |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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