SHORT COMMUNICATION: Photoreceptor rim protein: partial sequences of cDNA show a high degree of similarity to ABC transporters
| Autor: | Carolyn A. Converse, John V. Forrester, John E. Fothergill, James L. Thomson, Henry Brzeski, Bryan Dunbar |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
chemistry.chemical_classification
medicine.diagnostic_test Proteolysis ATP-binding cassette transporter Peptide Biology Molecular biology Sensory Systems law.invention Cellular and Molecular Neuroscience Ophthalmology Biochemistry chemistry law Complementary DNA medicine Primer (molecular biology) Peptide sequence Polymerase chain reaction Sequence (medicine) |
| Zdroj: | Current Eye Research. 16:741-745 |
| ISSN: | 1460-2202 0271-3683 |
| DOI: | 10.1076/ceyr.16.7.741.5060 |
| Popis: | PURPOSE. To isolate and sequence cDNA for bovine rim protein, a large membrane-bound glycoprotein found in photoreceptor outer segments. METHODS. Bovine rim protein was N-terminally sequenced (22 residues) and fragments were prepared by partial proteolysis. Two internal sequences of 21 and 18 amino acid residues were obtained from 35 kDa and 32 kDa fragments, respectively. Sense and anti-sense oligonucleotide primers were constructed, based on the peptide sequences derived from the 35 kDa and 32 kDa fragments, respectively, and the polymerase chain reaction (PCR) was used to amplify a 150 bp sequence from bovine retinal cDNA. RESULTS. The amplified sequence coded for the remainder of the peptide sequence determined from the 35 kDa fragment, which was not present in the primer, confirming that it was derived from the rim protein. The 150 bp sequence was translated to give a 50 amino acid peptide. Part of this peptide was compared with Dna sequence databases using the TFastA program, which found 94.6% ident... |
| Databáze: | OpenAIRE |
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