Aquatic reservoir of Vibrio cholerae in an African Great Lake assessed by large scale plankton sampling and ultrasensitive molecular methods
Autor: | Rowena Stern, Martina Brunetta, Alessio Borello, Ismael A. Kimirei, Elisa Taviani, Simona Coco, Luigi Vezzulli, Jaime Martinez-Urtaza, Andrés Santos, Luca Longo, Carla Pruzzo, Rita R. Colwell, Lance Gregory, William H. Wilson, Pierre Denis Plisnier, Caterina Oliveri |
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Rok vydání: | 2021 |
Předmět: |
0303 health sciences
Brackish water 030306 microbiology Ecology Lake ecosystem Sampling (statistics) General Medicine Plankton medicine.disease medicine.disease_cause Cholera 03 medical and health sciences Vibrio cholerae medicine Environmental science Transect Surface water 030304 developmental biology |
Zdroj: | ISME Communications. 1 |
ISSN: | 2730-6151 |
Popis: | The significance of large tropical lakes as environmental reservoirs of Vibrio cholerae in cholera endemic countries has yet to be established. By combining large scale plankton sampling, microbial culture and ultrasensitive molecular methods, namely Droplet Digital PCR (ddPCR) and targeted genomics, the presence of Vibrio cholerae was investigated in a 96,600 L volume of surface water collected on a 322 nautical mile (596 km) transect in Lake Tanganyika. V. cholerae was detected and identified in a large area of the lake. In contrast, toxigenic strains of V. cholerae O1 or O139 were not detected in plankton samples possibly in relation to environmental conditions of the lake ecosystem, namely very low salinity compared to marine brackish and coastal environments. This represents to our knowledge, the largest environmental study to determine the role of tropical lakes as a reservoir of V. cholerae. |
Databáze: | OpenAIRE |
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