Comparison of gene expression profiles in drug-withdrawn rats
| Autor: | Won-Keun Seong, Joon-Ik Ahn, Eun Jung Kim, Hye Jin Cha, Mun-Ji Choi, Seol-Hee Jeon, Ho-Sang Jeong, Hoil Kang, Hyung-Soo Kim |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Kainic acid Pentobarbital Microarray Health Toxicology and Mutagenesis Chloral hydrate Public Health Environmental and Occupational Health Neurotoxicity Physical dependence Long-term potentiation Pharmacology Toxicology medicine.disease Pathology and Forensic Medicine 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology chemistry Gene expression medicine General Pharmacology Toxicology and Pharmaceutics medicine.symptom medicine.drug |
| Zdroj: | Molecular & Cellular Toxicology. 12:197-207 |
| ISSN: | 2092-8467 1738-642X |
| Popis: | The aim of the present study was to compare gene expression profiles in animals treated with abusable drugs. Wistar rats were treated with saline, morphine, chloral hydrate, or pentobarbital twice daily for 14 days. To induce neurotoxicity, a group of rats was treated with kainic acid. 24 hr after withdrawal, and a genome-wide DNA microarray analysis was conducted with the striata. A total of 57 gene expression patterns were altered in the three test groups; 35 of the 157 genes were selected when compared with the kainic acid treated group. Twenty-two of the 24 genes were functionally described in the gene library, and many were involved in long-term potentiation (LTP) and cell adhesion. The results were confirmed by quantitative reverse transcription-polymerase chain reaction analysis. Taken together, the selected genes may subserve identification of physical dependence biomarkers and contribute to determining the molecular mechanisms underlying physical dependence. |
| Databáze: | OpenAIRE |
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