Gene expression in Nicotiana tabacum in response to compatible and incompatible isolates of Pseudomonas solanacearum
| Autor: | Fatima Ragueh, Yves Marco, Dominique Roby, Nicole Lescure |
|---|---|
| Rok vydání: | 1989 |
| Předmět: |
0106 biological sciences
Hypersensitive response 0303 health sciences biology Inoculation Bacterial wilt Nicotiana tabacum Pseudomonas food and beverages Plant Science biology.organism_classification 01 natural sciences Microbiology 03 medical and health sciences Gene expression Genetics Solanaceae 030304 developmental biology 010606 plant biology & botany Pseudomonadaceae |
| Zdroj: | Physiological and Molecular Plant Pathology. 35:23-33 |
| ISSN: | 0885-5765 |
| DOI: | 10.1016/0885-5765(89)90004-0 |
| Popis: | We report changes in gene expression in Nicotiana tabacum after inoculation with compatible and incompatible strains of Pseudomonas solanacearum, the causative agent of bacterial wilt of several economically important Solanaceae. Three isolates of P. solanacearum were used: GMI1000, (incompatible), which elicits the hypersensitive response (HR) in tobacco leaves; K60, (compatible), which induces the development of the disease in the same host; and GMI1178, a deletion mutant of GMI1000, which has lost its ability to produce any symptoms in inoculated plants. Total and poly(A)+ RNAs prepared from leaves infiltrated with these different isolates or with water, were translated in a rabbit reticulocyte lysate. Comparison of the polypeptide products by two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) indicated different effects of these treatments on plant transcription. Whereas water had little influence on the plant gene expression, GMI1178 and K60 each caused the appearance of similar new transcripts in the infiltrated leaves, 6 h after inoculation. Further changes in the mRNA species were observed in leaves infected with K60, 12 to 18 h after inoculation. However, pronounced differences were already visible 6 h after infiltration of tobacco leaves with GMI1000. Our results indicate that distinct molecular events are associated with the hypersensitive response. |
| Databáze: | OpenAIRE |
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