Construction of OVA-stabilized fluorescent gold nanoclusters for sensing glucose

Autor: Juan Qiao, Li Qi, Xiaozhe Xu, Dan Li, Lu-Liang Wang
Rok vydání: 2015
Předmět:
Zdroj: Science China Chemistry. 58:1508-1514
ISSN: 1869-1870
1674-7291
Popis: Protein protected gold nanoclusters have outstanding physical and chemical properties that make them excellent scaffolds for the construction of novel chemical and biological probes. In this study, a simple one-pot synthesis method was proposed for the preparation of fluorescent probes based on ovalbumin-stabilized gold nanoclusters. This strategy allowed the generation of water-soluble gold nanoclusters within 5 min. The as-prepared fluorescent probe exhibited a red fluorescence emission at 625 nm, and good thermostability. The fluorescent probe was applied to measure glucose concentrations based on the hydrogen peroxide-induced fluorescence quenching principle, and showed favorable biocompatibility, high sensitivity and good selectivity. As a result of the advantageous properties and performance of this fluorescent probe, the present assay allowed for the selective determination of glucose in the range of 5.0x10(-6) to 10.0x10(-3) mol/L with a detection limit of 1.0x10(-6) mol/L. Moreover, the glucose content in urinary samples was analyzed using the constructed fluorescent probe: this indicated the potential of the fluorescent gold nanoclusters for applications in biological and clinical diagnosis and therapy.
Databáze: OpenAIRE
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