Production and characterization of intergeneric hybrids between Brassica oleracea and a wild relative Moricandia arvensis

Autor: Yasuo Matsuzawa, E. Satake, S. W. Bang, Y. Kaneko, R. Kaneko, K. Sugihara, B. H. Jeung
Rok vydání: 2007
Předmět:
Zdroj: Plant Breeding. 126:101-103
ISSN: 1439-0523
0179-9541
DOI: 10.1111/j.1439-0523.2007.01307.x
Popis: Intergeneric crosses were made between Brassica oleracea and Moricandia arvensis utilizing embryo rescue. Six F 1 hybrid plants were generated in the cross-combination of B. oleracea x M. arvensis from 64 pods by the placenta-embryo culture technique, whereas three plants were produced in the reciprocal cross from 40 pods by the ovary culture technique. The hybrid plants- were ascertained to be amphihaploid with 2n = 23 chromosomes in mitosis and a meiotic chromosome association of (0-3)11 + (17-23)1 at metaphase I (M I). In the backcross with B. oleracea, some of these hybrids developed sesquidiploid BC 1 plants with 2n = 32 chromosomes that predominantly exhibited a meiotic configuration of (911 + 14I) in pollen mother cells. The following backcross of BC 2 plants to B. oleracea generated 48 BC 3 progeny with somatic chromosomes from 2n = 19 to 2n = 41. The 2n = 19 plants showed a chromosomal association type of (9II + 1I) and a chromosomal distribution type of (91/2 + 91/2) or (9 + 10) at M I and M II, respectively. These facts might suggest that they were monosomic addition lines (MALs) of B. oleracea carrying a single chromosome of M. arvensis that could offer potential for future genetic and breeding research, together with other novel hybrid progeny developed in this intergeneric hybridization.
Databáze: OpenAIRE
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