Quantitative Cytochrome Oxidase Histochemistry: Applications in Human Alzheimer's Disease and Animal Models
| Autor: | Francisco Gonzalez-Lima, Jon Valla, Alison M. Small, Lonnie Schneider |
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| Rok vydání: | 2007 |
| Předmět: | |
| Zdroj: | Journal of Histotechnology. 30:235-247 |
| ISSN: | 2046-0236 0147-8885 |
| DOI: | 10.1179/his.2007.30.4.235 |
| Popis: | Quantitative cytochrome oxidase (CO) histochemistry provides an intracellular measure of oxidative energy meta bolic capacity that can be used as a tool to evaluate brain functional changes. CO enzyme activity also has been shown to be catalytically abnormal in patients with Alzhei mer's disease (AD). The application of CO histochemistry to postmortem AD and control human brains, as well as in comparative studies of murine models, is presented here, from the cellular to laminar to regional and multiregional levels of analysis. In AD cases and controls, we compared CO in I 1 brain regions-of-interest (ROIs), including lami nar analyses in seven neocortical regions. In a transgenic mouse model of AD and controls, we compared CO in 56 ROIs, providing information about both regional and net work activity. Results highlight cellular and laminar vulner abilities in AD and illustrate homologies between AD cases and a murine model of AD, with age-related reductions in CO activity within circuits implicated in spatial and dis criminative learning. Quantitative CO histochemistr y is a useful tool for the ex vivo assessment of functional changes within brain regions and neural networks, and its applica tion has provided support for the hypothesis that mitochon drial dysfunction is involved in the pathophysiology of AD. (The J Histotechnol 30:235, 2007) |
| Databáze: | OpenAIRE |
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