The Corn Inhibitor of Blood Coagulation Factor XIIa
| Autor: | Maryam Hazegh-Azam, Lars C. Pedersen, George von Dassow, Gerald R. Reeck, Vivien C. Yee, David C. Teller, Ronald E. Stenkamp |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
chemistry.chemical_classification
HEPES biology Factor XIIa Polyethylene glycol Protease inhibitor (biology) law.invention Solvent chemistry.chemical_compound Crystallography Enzyme chemistry Structural Biology Enzyme inhibitor law biology.protein medicine Crystallization Molecular Biology medicine.drug |
| Zdroj: | Journal of Molecular Biology. 236:385-387 |
| ISSN: | 0022-2836 |
| DOI: | 10.1006/jmbi.1994.1147 |
| Popis: | A 13.6 kDa protein from corn seeds is known to be a highly selective inhibitor of human blood coagulation Factor XIIa (or activated Hageman factor). We have crystallized this inhibitor at 23 degrees C and pH 7.5 from a solution of 30% polyethylene glycol 400, 0.2 M MgCl2, and 0.1 M Hepes. The crystals diffract to at least 2.1 A resolution. The space group is P4(2)2(1)2 with a = b = 57.15 A and c = 80.5 A. The crystals contain 51% solvent. Two heavy atom derivatives have been identified. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|