RNase I Modulates Escherichia coli Motility, Metabolism, and Resistance
| Autor: | Yashasvika Duggal, Gang Ning, Deanna M. Dailey, Benjamin M. Fontaine, Emily E. Weinert |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Cell signaling 010405 organic chemistry Chemistry RNase P Endoribonuclease RNA Motility General Medicine medicine.disease_cause 01 natural sciences Biochemistry 0104 chemical sciences Cell biology 03 medical and health sciences 030104 developmental biology Second messenger system medicine Molecular Medicine Bacterial outer membrane Escherichia coli |
| Zdroj: | ACS Chemical Biology. 15:1996-2004 |
| ISSN: | 1554-8937 1554-8929 |
| DOI: | 10.1021/acschembio.0c00390 |
| Popis: | Bacteria are constantly adapting to their environment by sensing extracellular factors that trigger production of intracellular signaling molecules, known as second messengers. Recently, 2',3'-cyclic nucleotide monophosphates (2',3'-cNMPs) were identified in Escherichia coli and have emerged as possible novel signaling molecules. 2',3'-cNMPs are produced through endonucleolytic cleavage of short RNAs by the T2 endoribonuclease, RNase I; however, the physiological roles of RNase I remain unclear. Our transcriptomic analysis suggests that RNase I is involved in modulating numerous cellular processes, including nucleotide metabolism, motility, acid sensitivity, metal homeostasis, and outer membrane morphology. Through a combination of deletion strain and inhibitor studies, we demonstrate that RNase I plays a previously unknown role in E. coli stress resistance by affecting pathways that are part of the defense mechanisms employed by bacteria when introduced to external threats, including antibiotics. Thus, this work provides insight into the emerging roles of RNase I in bacterial signaling and physiology and highlights the potential of RNase I as a target for antibacterial adjuvants. |
| Databáze: | OpenAIRE |
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